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The overall aims of your division of Experimental Hematology are to acquire comprehensive expertise on (molecular) mechanisms that identify hematopoietic stem mobile self-renewal and differentiation, with the ultimate purpose to deepen our insights in the event of human leukemias. We've a specific interest in dissecting the clonal heterogeneity of leukemias and not long ago produced instruments that make it possible for us to establish and prospectively isolate genetically distinctive clones within individual patients. We complete gene-operate analyses in human hematopoietic stem and progenitor cells isolated from cordblood and bone marrow using different techniques like optimized retro/lentiviral transduction protocols and inducible RNAi and CRISPR/Cas9 strategies. We have a longstanding expertise in using molecular strategies (transcriptome, proteome and epigenome) to even further fully grasp processes like hematopoietic differentiation, proliferation, apoptosis and self-renewal.

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Downregulation of RAC2 or SAM50 impaired self-renewal associated with a lessened mitochondrial membrane opportunity and impaired mitochondrial integrity as determined by EM.

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